SOP for the Proper Technique for Withdraw of Liquids from Multiuse Vials or Bags
This SOP is a companion to Animal User Requirement #5, “Determining Expiration Dates for Substances Used in Research Animals,” and it applies to all personnel involved in activities that use live vertebrate animals for biomedical research and/or teaching purposes at UW-Madison facilities regardless of the funding source.
- Wash hands or put on clean exam gloves.
- Assemble needle and syringe.
- Check the label to make sure you have the correct substance/fluid type.
- Check the expiration date on the vial/fluid bag.
- Do not use if past expiration date.
- Look at the vial/ fluid bag and check for:
- Change in color
- Small pieces floating in the liquid
If any of these are present discard the fluid or check with knowledgeable personnel before giving the injection.
- If this is the first time using the vial, remove cap or for fluids remove from protective pouch.
- Wipe the rubber top (septum) with alcohol.
- With the cap still on the needle, pull back on the plunger to the line on your syringe for your dose.
- This will fill the syringe with air
- Carefully remove the cap from the needle.
- Insert the needle into the rubber top (septum) of the vial/fluid bag. Do not touch or bend the needle.
- Push the air into the vial/fluid bag.
- This helps prevent a vacuum from forming when you try to withdraw the fluid.
- Turn the vial upside down or position the bag so that the fluid is above the needle inside the bag.
- Pull back on the plunger to withdraw the necessary fluid amount.
- If needed gently tap on the barrel of the syringe to force air bubbles into the hub of the needle.
- Push the plunger to put the air back into the vial/bag, and than carefully re-withdraw to the required fluid amount.
- Remove the syringe and needle from the vial/bag and give the injection.
- Dispose of the uncapped syringe and needle in the sharps container.