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SMPH Policy Number: 14

Policy Title: Oocyte Harvest in Frogs

Policy: Role of the Investigator:

The investigator must include a written description in the animal protocol of the proposed procedures, and ensure that their laboratory staff follows the approved procedures.


Role of the Veterinary Staff and Biomedical Research Model Services:

If any animal develops unexpected clinical complications, including death, the veterinary staff must be notified in a timely way. Diagnosis and treatment of unanticipated complications are the responsibility of the attending veterinarian in consultation with the investigator. Animals that die unexpectedly during or after surgery or are euthanized because of post-surgical complications should be submitted for necropsy. Postmortem examinations may be performed at the discretion of the veterinary staff at no charge to the investigator.


ACUC Oversight:

The ACUC shall review proposed protocols for conformity to these guidelines, and enforce compliance.


References:

Oocyte Harvesting in Xenopus Laevis. 2001 revision. ARAC Guidelines

Oocyte Surgery

Green, S.L. Overview: Factors Affecting Oogenesis in the South African Clawed Frog (Xenopus laevis). Comparative Medicine 2002 August; 52(4): 307-312


History: Approved: August 2005

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